Welcome to the Raj Laboratory
Our lab is interested in building a quantitative understanding of cellular function. We like to develop new tools for quantifying biological processes based on imaging and sequencing and then use those techniques to help us answer questions in molecular and cellular biology.
We aim to foster a richly interdisciplinary research environment in which biologists, chemists, engineers, clinicians, physicists, computer scientists and mathematicians can work together to solve exciting problems. Contact me if you are interested in joining the lab.
Some recent topics of interest include:
- Chromosome structure and gene expression
- Per-chromosome transcriptional regulation
- Non-coding RNA
- Homeostatic mechanisms in the regulation of biomolecules
- Single cell and single chromosome analysis of gene expression in cancer
- Non-coding RNA
One of the tools we use in our research is an in situ hybridization method (RNA FISH) that enables us to visualize, count and localize individual RNA molecules in single cells. We are continuously refining and expanding our RNA visualization toolkit, and are also looking to combine the precision of imaging with the power of sequencing. Biologically, we are interested in a variety of topics including those above. Stay tuned for more information in the coming months!
Also, check out our lab blog, which is a bit more up to date with the latest from the lab (scientific and otherwise).
If you are interested in getting started with single molecule RNA FISH yourself, check out this website. It has a lot of information and answers to many common questions.
If you want to count your spots, use this software. It is easy to use and gives good results.
Our work is funded by generous support from the NIH New Innovator Program and the Burroughs-Wellcome Fund.